29 5 月 Phone viability: Mobile stability is reviewed of the MTT assay within the HEK Blue hTLR2 cells because the in earlier times demonstrated

14 10 % (v/v) DMSO (Carl Roth) served once the handle as well as the viability out of untreated cells was discussed as the 100 %.

Statistical analysis: Data of the bar charts are shown as mean+SD. Potency (IC_fifty) data are presented as mean with the confidence interval (95 %). Statistical analysis was done by using GraphPad Prism six.0 (GraphPad software, San Diego, USA). Nonlinear regression was used to plot and analyze concentration-response curves and to obtain IC₅₀ values.

Computational methods: The crystal structure of the heterodimer of TLR2-TLR1 with bound Pam₃CSK₄ (PDB ID: 2Z7X) 15 was retrieved from the Protein Data Bank sixteen and used for docking studies with the tested compounds. Prior to docking the TLR1 monomer, all ligands and water molecules were removed using Molecular Operating Environment (MOE2019, Chemical Computing Group, Montreal, QC, Canada).